Production Methods Research Articles

Non-Covalent Cross-Linking Hydrogel: A New Method for Visceral Hemostasis

Excessive blood loss could lead to pathological conditions such as tissue necrosis, organ failure, and death. The limitations of recently developed hemostatic approaches, such as their low mechanical strength, inadequate wet tissue adhesion, and weak hemostatic activity, pose challenges for their application in controlling visceral bleeding. In this study, a novel hydrogel (CT) made of collagen and tannic acid (TA) was proposed. By altering the proportions between the two materials, the mechanical properties, adhesion, and coagulation ability were evaluated. Compared to commercial hydrogels, this hydrogel has shown reduced blood loss and shorter hemostatic time in rat hepatic and cardiac bleeding models. This was explained by the hydrogel’s natural hemostatic properties and the significant benefits of wound closure in a moist environment. Better biodegradability was achieved through the non-covalent connection between tannic acid and collagen, allowing for hemostasis without hindering subsequent tissue repair. Therefore, this hydrogel is a new method for visceral hemostasis that offers significant advantages in treating acute wounds and controlling major bleeding. And the production method is simple and efficient, which facilitates its translation to clinical applications.

The Impact of COVID-19 on Consumer Perceptions of Local Food Market Channels

The COVID-19 pandemic had a profound impact on food systems. Despite disrupting conventional markets—such as grocery stores—farmers that focused on short supply chains found new market opportunities. As consumers increasingly became concerned with the safety of in-person shopping, some turned to purchasing directly from farmers and markets that carried products marketed as local, organic, and/or sustainable. With these changes in mind, I ask how consumer perceptions of local food systems (LFSs) changed in the two years after COVID-19. If consumers are more aware of local markets, farmers might reorient production to focus on local supply chains and sustainable production methods. To evaluate consumer perceptions, I use survey responses from residents in five communities in the US South in 2019 and 2022. Using a treatment effects model with propensity score matching, I show that residents became more aware of most local market channels, such as specialty retail stores and Community Supported Agriculture (CSA). Some residents were also more likely to have a positive perception of markets that provide fresh vegetables to low-income residents. Finally, residents wanted more investment into markets that make fresh, local products more available, such as community-owned groceries and CSA.

Exploring the potential of ammonia and hydrogen as alternative fuels for transportation

Abstract This study investigates the potential of hydrogen and ammonia, as alternatives for transportation fuels to tackle urgent issues concerning greenhouse gas emissions and air quality in the worldwide transportation sector. By examining studies and technological progresses, we evaluate the feasibility of transitioning to these energy options. Through an investigation of production methods, energy efficiency, environmental consequences, and infrastructure requirements, we present both the advantages and disadvantages of using hydrogen and ammonia as fuel substitutes. We spotlight production techniques such as electrolysis and renewable energy sources that could significantly decrease carbon emissions and air pollutants. Nonetheless, key challenges such as expanding infrastructure, cost-effectiveness, and safety considerations need to be resolved for adoption. Drawing on findings from research and industry developments, this article contributes to publications on transportation solutions while proposing avenues for research efforts and policy initiatives. Existing challenges and limitations are also discussed in details. In conclusion, this research underscores the significance of research endeavors and policy backing to unlock the potential of hydrogen and ammonia as sustainable transportation fuels underscoring their role in mitigating environmental impacts and promoting global sustainability objectives.

Design of intelligent agricultural landscape planning and ecological balance control system based on remote sensing images

Abstract With the modernization of agricultural production and the acceleration of urbanization, agricultural landscape planning and ecological balance control have become important issues in promoting sustainable development. Traditional agricultural production methods have problems such as resource waste, environmental pollution, and ecological damage. This article aimed to achieve scientific planning and management of agricultural landscapes, and promote the efficiency of agricultural production, rational utilization of resources, and protection of the ecological environment, thereby promoting sustainable agricultural development. This study first focused on denoising and feature extraction of agricultural remote sensing data images, using remote sensing technology to obtain agricultural landscape information. After that, artificial intelligence was adopted to achieve intelligent agricultural landscape planning, with the goal of maintaining ecological balance and promoting efficient, eco-friendly, and sustainable agricultural production. This study took the landscape of a certain city as an example to test the processing effect of remote sensing images, and it was proved that the effect was good. We tested the performance of an intelligent agricultural landscape planning and ecological balance control system based on remote sensing images. According to the experimental results, it can be concluded that the resource utilization efficiency of farmland A monitored by the intelligent agricultural landscape planning system using remote sensing image technology was 82%, while the traditional one was only 60%. This indicated that the collection effect of remote sensing image technology was much better than that of traditional technology. This article comprehensively and timely monitored and evaluated farmland through remote sensing image technology, including crop growth status, soil moisture, nutritional status, etc. The system can provide precise planting and management suggestions for farmers based on this information, and help them optimize farmland layout, crop selection, and irrigation management, thereby improving the production efficiency of farmland and crop yield.

Obesity-Related Traits Mediate the Effects of Educational Attainment on the Risk of Varicose Veins, Venous Thromboembolism, and Phlebitis.

The extent to which educational attainment (EA) influences the risk of varicose veins (VVs), venous thromboembolism (VTE), and phlebitis occurrence, whether this pathway is mediated by obesity-related traits, and the proportion of their mediation is unknown. A Mendelian randomization (MR) design was used to genetically investigate the causal effects of EA on the risk of VV, VTE, and phlebitis and to assess the mediating effect of obesity-related traits. Causal effects were estimated using primarily the multiplicative random-effects inverse variance-weighted method. This was supplemented by Cochran's Q-statistic, MR-Egger regression, MR funnel plots, and leave-one-out test to evaluate the reliability of the results. For the individual mediation effect, the coefficient product method was mainly utilized to estimate. An increase in genetically predicted EA was associated with a lower risk of VV, VTE, and phlebitis, as well as lower body mass index, basal metabolic rate, hip circumference, and waist circumference. As genetically predicted body mass index, basal metabolic rate, hip circumference, and waist circumference increased, the risk of developing VV, VTE, and phlebitis increased, respectively. Body mass index, basal metabolic rate, hip circumference, and waist circumference were identified as mediators of the protective effects of EA on VV, VTE, and phlebitis. The findings support a causal relationship between higher EA and lower risk of VV, VTE, and phlebitis. Obesity-related traits play a significant mediating role in these pathways, and there are interactions between them, with hip circumference mediating these pathways relatively independently from the other three.

Advancements in Biochar Modification for Enhanced Phosphorus Utilization in Agriculture

The role of modified biochar in enhancing phosphorus (P) availability is gaining attention as an environmentally friendly approach to address soil P deficiency, a global agricultural challenge. Traditional phosphatic fertilizers, while essential for crop yield, are costly and environmentally detrimental owing to P fixation and leaching. Modified biochar presents a promising alternative with improved properties such as increased porosity, surface area, and cation exchange capacity. This review delves into the variability of biochar properties based on source and production methods and how these can be optimized for effective P adsorption. By adjusting properties such as pH levels and functional groups to align with the phosphate’s zero point of charge, we enhance biochar’s ability to adsorb and retain P, thereby increasing its bioavailability to plants. The integration of nanotechnology and advanced characterization techniques aids in understanding the structural nuances of biochar and its interactions with phosphorus. This approach offers multiple benefits: it enables farmers to use phosphorus more efficiently, reducing the need for traditional fertilizers and thereby minimizing environmental impacts, such as greenhouse gas emissions and P leaching. This review also identifies existing research gaps and future opportunities for further biochar modifications. These findings emphasize the significant potential of modified biochar in sustainable agriculture.

Analysis of the Impact of Globalization Trends in the Digital Economy on Business Management and Administration Systems of Enterprises

Aim. This study aims to investigate the impact of globalization trends on the business management and administration systems. Methodology. The scoping review of the literature included 123 articles through desk research and in-house material. Upon categorizing typologies as qualitative, quantitative, or mixed, an assessment was made regarding the performance and consequences of globalization from 1990 to 2022. Results. Developing country managers are more likely to depend on international sales as a means to augment revenue, due to the greater saturation of sales in developed markets. Developed economies focus on advanced, possibly automated production methods, whereas developing nations employ the digital economy to compete in less automated industries. This creates a more specialized global market..E-commerce in the digital economy makes it easier for developing nations to enter the global market and reach a global audience. In their pursuit of financial gain, corporations based in affluent nations are more likely to employ sophisticated production methods, while those situated in developing countries are more likely to utilize less advanced ones. A number of prevalent issues and trends—including economics, politics, emerging markets, corporate social responsibility, and emerging markets—were recognized as critical for the development of global markets. While past research has focused on globalization's effects on developed economies, this study examines emerging country managers' unique difficulties and opportunities. The emphasis on expanding international sales and potential manufacturing process reduction can inform management approaches in similar situations. Conclusion. The recommendations underscore the critical necessity for further investigation into the aforementioned impacts across diverse economies, ranging from developing to developed. Although this study encountered difficulties due to inconsistent and limited data, it established a strong foundation for subsequent investigations in the field through the application of operationalization, methodologies, and analyses discovered in the business literature.

Analysis of large-scale high-quality graphene production and applications

Graphene, a single-atom-thick layer of carbon atoms arranged in a hexagonal lattice, is the thinnest and strongest material known to mankind. It has excellent electrical, thermal, and mechanical properties, making it a promising material for a wide range of applications in electronics, optoelectronics, energy storage, and more. With the increasing demand for graphene in various applications, large-scale and high-quality graphene production has become a significant challenge. While early methods of graphene production involved mechanical exfoliation, this method is limited in terms of scalability and yield. To meet the increasing demand for large-scale production of graphene, various methods have been developed in recent years, including chemical vapor precipitation, epitaxial crystal growth, graphene oxide reduction and solvent exfoliation and so on. This study aims to introduce several existing methods for the mass production of graphene with high quality and analyzes the advantages and disadvantage involve thereof. The findings in this paper may provide a valuable reference for the industrial-scale production of graphene.

An Overview of Hydrothermally Synthesized Titanate Nanotubes: The Factors Affecting Preparation and Their Promising Pharmaceutical Applications

Recently, titanate nanotubes (TNTs) have been receiving more attention and becoming an attractive candidate for use in several disciplines. With their promising results and outstanding performance, they bring added value to any field using them, such as green chemistry, engineering, and medicine. Their good biocompatibility, high resistance, and special physicochemical properties also provide a wide spectrum of advantages that could be of crucial importance for investment in different platforms, especially medical and pharmaceutical ones. Hydrothermal treatment is one of the most popular methods for TNT preparation because it is a simple, cost-effective, and environmentally friendly water-based procedure. It is also considered as a strong candidate for large-scale production intended for biomedical application because of its high yield and the special properties of the resulting nanotubes, especially their small diameters, which are more appropriate for drug delivery and long circulation. TNTs’ properties highly differ according to the preparation conditions, which would later affect their subsequent application field. The aim of this review is to discuss the factors that could possibly affect their synthesis and determine the transformations that could happen according to the variation of factors. To fulfil this aim, relevant scientific databases (Web of Science, Scopus, PubMed, etc.) were searched using the keywords titanate nanotubes, hydrothermal treatment, synthesis, temperature, time, alkaline medium, post treatment, acid washing, calcination, pharmaceutical applications, drug delivery, etc. The articles discussing TNTs preparation by hydrothermal synthesis were selected, and papers discussing other preparation methods were excluded; then, the results were evaluated based on a careful reading of the selected articles. This investigation and comprehensive review of different parameters could be the answer to several problems concerning establishing a producible method of TNTs production, and it might also help to optimize their characteristics and then extend their application limits to further domains that are not yet totally revealed, especially the pharmaceutical industry and drug delivery.

The Internet Doesn't Exist in the Sky: Literacy, AI, and the Digital Middle Passage

AbstractThis article utilizes speculative and visual storytelling alongside interdisciplinary research on artificial intelligence (AI) and algorithmic oppression to engage in a thought experiment on how literacy studies might refuse the oppressionist logics currently undermining the possibilities of AI in literacy education. As technological advancements in education will only continue to increase and as society is yet to ascertain the parameters of an ethical AI system, it is paramount to analyze the past and present and contemplate potential futures, especially those that do not result in violence against Black and Brown peoples. To engage in speculation, we employ Endarkened Storywork (Toliver, 2022) to present an empirically driven, futuristic, science fiction narrative from two perspectives: (1) a US, Black girl who is forced to participate in AI‐structured secondary schooling and (2) a Black girl in Haiti who is forced to live in a country polluted by technological byproduct. This narrative, which is grounded in academic research and news editorials, is accompanied by comic art and followed by a companion analysis detailing the theoretical backdrop of the story. By utilizing multiple methods of scholarly distribution, we provide multiple entry points for readers to engage with this work. We offer a means for readers to see—via story, art, and scholarship—the potential impacts of AI on Black people globally. Additionally, by situating this article in the creative and scholarly realms, we strategically deconstruct traditional forms and methods of knowledge production that have constrained academic research and rendered invisible alternative forms of data representation.

Assessment of Safe Vegetable Cultivation at Jashore in Bangladesh: Evidence from Country Bean, Cucumber and Pointed Gourd Cultivation

The study was designed to assess the present status and methods of safe vegetable production at farm level in selected areas of Jashore during January-May, 2023. For the study ninety sample farmer were selected by stratified sampling from three sub-district of Jashore. Average farm size was 0.79 ha in study area. Farmer doing different agronomic management such as weeding, irrigation, spraying, de-topping, stalking, earthing up etc. Farmer used different methods simultaneously such as insecticide spray, bio-pesticide, sex pheromone, yellow trap, white trap, hand picking etc. for controlling insect pest. About sixty-four percent farmer sprayed synthetic pesticide and rest of them used bio control method such as bio-pesticide (bio-chamak, eco-mac, bio-envir, bio-cin), sex pheromone trap, yellow trap and hand picking for control insect pest in their fields. About fifty-eight percent farmer used bio-fertilizer along with organic fertilizer (manure, vermin compost) and seventy-eight percent farmer used chemical fertilizer for vegetable cultivation. Total production cost of country bean was Tk.3,36,579 ha-1, cucumber was Tk.3,25,915 ha-1 and pointed gourd was Tk.4,40,106 ha-1. Among the cost item labor cost was highest. Average yield of country bean was 30.60 tha-1, cucumber was 30.08 tha-1 and pointed gourd was 41.60 tha-1. Highest benefit cost ratio was cucumber (2.13) followed by pointed gourd (2.07) and country bean (1.50), that means safe vegetable cultivation also profitable. Farmer awareness, havoc attack of pest and insect were the main problem for safe vegetable cultivation. Market facilities, demand, value chain, proper price for organic vegetable, and farmer motivation can promote and enhance safe vegetable cultivation.

Bridging the gap: integrating AR and VR production line training to enhance furniture carpentry students’ understanding of production processes

ABSTRACT Large-scale furniture production involves complex manufacturing processes and methods. However, Taiwanese schools face limitations in terms of space and equipment, restricting students from engaging in furniture prototype creation or small-batch production. As a result, students often struggle to grasp practical aspects of large-scale furniture production, leading to a partial understanding of manufacturing processes and methods. To bridge this gap, this study introduces Augmented Reality (AR) technology and miniaturized furniture production lines in a school’s woodshop. AR can be used to guide students through miniaturized production stages, emphasizing semi-automated furniture production features. Additionally, Virtual Reality (VR) technology is combined to provide an immersive experience of real-world, large-scale furniture production, allowing students to understand processing scenarios and conditions in facilities of other countries. In an experiment involving 30 first-year students, an experimental group (15 participants) received mixed AR and VR instruction, while a control group (15 participants) received traditional teaching methods. Findings reveal that AR and VR integration enhanced students’ comprehension of differences between the school workship and overseas large-scale furniture production facilities. This strategy improved their furniture production knowledge, and fostered adaptability to various production conditions and methods.

Experimental study and numerical simulation on porosity dependent direct reducibility of high-grade iron oxide pellets in hydrogen

The transition to more environmentally friendly steel production methods has intensified research into hydrogen-based direct reduction (HyDR) of iron oxide pellets. The aim of this study is to systematically investigate the kinetics of the reduction process, the evolution of porosity and the resulting microstructural changes on the reduction behavior of high-quality pellets during HyDR of iron ore at different temperatures. A modified mathematical model is developed based on the shrinkage kernel model, taking into account both mass and heat transport in a hydrogen atmosphere. The effects of temperature, particle size and time on the reduction behavior of the pellets are investigated. The simulated results are validated and discussed by the results of a batch of iron oxide pellets consisting of ten almost spherical pellets subjected to the direct reduction process with pure hydrogen. The results show that the total energy input to the HyDR process is a complex balance of factors, including chemical reaction rates, diffusion dynamics and entropy generation. The increase in free volume and simultaneous decrease in pore diameter reflect the dynamic nature of the microstructure, which includes additional free volume and defects due to the volume discrepancies and associated stresses between the reactant and product phases. Furthermore, the data show that higher temperatures accelerate the reduction reactions, especially the transformation of wustite into metallic iron. This phase transition is characterized by a significant volume change that cannot be accommodated by elastic deformation alone, leading to the development of lattice defects such as cracks, creep pores and dislocations that serve as stress relief mechanisms. The trends for porosity change at 950 °C and 1000 °C observed in the experimental results are correct and in good agreement with the numerical and simulated results.

China’s ecological footprint via biomass import and consumption is increasing

As China’s economic and population demands grow, the need for biomass imports rises, presenting challenges for sustainable development and global ecosystem protection. Here we examine China’s increasing influence on global ecosystems through its biomass product consumption and trade using the Human Appropriation of Net Primary Productivity framework, analyzing data from 2004 to 2017 with projections until 2050. In 2017, China was the world’s largest biomass consumer, consuming approximately 1.75 petagrams of carbon per year, projected to rise by 317.6% by 2050, highlighting China’s major role in global ecosystem stress. Our findings also show an increase in biomass imports from lower-middle-income and low-income countries between 2004 and 2017, and these imports are expected to continue increasing by 402.9% in 2050. The analysis reveals that domestic product demand and changes in foreign production efficiency are key drivers of this trend, suggesting the need for China to shift towards more efficient trade practices and support cleaner production methods internationally.

Fungal mycelium-based biofoam composite: A review in growth, properties and application

Recent years have shown a surge in interest in incorporating living systems into materials research to synthesize functional materials using biological resources. Among these, mycelium-based materials, notably biofoam, have emerged as innovative solutions for repurposing organic wastes that were previously considered unusable. The growth of mycelium, vital for the synthesis of biofoam, is influenced by a multuple of factors including substrate composition, moisture content, temperature, nutrient availability, pH levels, oxygen concentration, and measures for contamination control. Additionally, physical stimulation techniques have been explored to enhance mycelium growth, ranging from cold stress-induced adaptations to electrical shock-induced modifications and optimization of sound treatments and light exposure. This review highlights the growing interest in biofoam composite materials, a novel class of environmentally friendly and cost-effective materials that are gaining popularity, for advancing sustainable construction practices. Biofoam composites use organic fungal growth as a low-energy bio-fabrication process to transform abundant agricultural byproducts and waste into viable alternatives to energy-intensive manufactured building materials. Their versatility in composition and manufacturing methods allows them to be used in a wide range of applications, including insulation and door cores, panelling, flooring, and furniture components. Notably, biofoams outperform synthetic foams and engineered wood in terms of thermal insulation, sound absorption, and fire resistance, making them highly promising for construction industry. Besides, due to its customizable composition and production method, biofoam can be used in the replacement of foams, leather, wood, and plastics in a variety of applications such as water treatment and filtration, medical supplies and healthcare applications. However, despite their remarkable properties, biofoam typically serve as non- or semi-structural supplements to traditional construction materials due to inherent limitations. Nevertheless, the useful material properties of these materials, combined with their low cost, ease of manufacture, and environmental sustainability, imply that they will have an important part to play in the development of environmentally friendly materials in the future.

Optimizing photosynthetic photon flux density and light quality for maximizing space use efficacy in edamame at the vegetative growth stage

Compared with conventional crop cultivation in greenhouses or fields, plant factories with artificial light (PFAL) have advantages in the highly efficient use of space, energy, and resources available for cultivation. However, few studies on environmental controls for improving the space use efficacy (SUE) of PFAL in the production of edamame, a vegetable soybean, have been reported. Therefore, developing an environmental control method for high productivity with minimal space and energy requirements is of high priority. The aims of this study were to (1) identify the optimal photosynthetic photon flux density (PPFD) and light quality to enhance the SUE of edamame at the vegetative growth stage, and (2) examine the effects of PPFD, light quality, and their interaction on edamame plant growth at the vegetative stage. SUE is defined as the crop biomass produced per unit cubic volume of cultivation during the growth period. We examined three PPFD treatments (300, 500, and 700 μmol m−2 s−1) with three color temperature LED lamps (3,000, 5,000, and 6,500 K), for a total of nine treatments. The results demonstrated that, under the same light quality treatment, higher PPFDs resulted in larger fresh and dry weights of all organs, higher stem length, and lower specific leaf area. Under the same PPFD treatment, a high ratio of blue (400–499 nm) to red (600–699 nm) photon flux density increased the plant height but decreased the projected leaf area. The values of SUE at 700 μmol m−2 s−1 increased by 213, 163, and 92% with 3,000, 5,000, and 6,500 K, respectively compared with those at 300 μmol m−2 s−1. The values of SUE at 700 μmol m−2 s−1 increased by 34 and 23% in 5,000 and 6,500 K treatments, respectively compared with that in the 3,000 K treatment. In conclusion, a combination of 700 μmol m−2 s−1 PPFD and 5,000 K color temperature is the suitable condition to increase the SUE of edamame at the vegetative growth stage in a PFAL.

A literature review on the planning and analysis of accelerated degradation testing for mechanical products

High reliability and long life are typical requirements for mechanical products. The performance requirements of mechanical products are increasing as science and technology develop, and the environment and working stress they bear are becoming more diverse. This issue has become one of the bottlenecks for mechanical products in lifespan and reliability generally. The aim of this study is to categorize, examine, and review the most applicable accelerated testing methods for mechanical products. The research status of accelerated modeling methods, statistical analysis, and design optimization of accelerated degradation testing(ADT) technology are reviewed. On the basis of literature analysis, the deficiencies and possible development directions of ADT were summarized and prospected. This work is anticipated to serve as a reference and provide important insights into the reliability of mechanical products.

Heavy metal application of response surface optimized-lipopeptide biosurfactant produced by Pseudomonas aeruginosa strain CGA-02 in low-cost substrate

Cost-effective methods of biosurfactant production with minimal environmental impact are needed as global demand continues to increase. This study evaluated lipopeptide biosurfactant production in a Pseudomonas aeruginosa strain CGA-02 using a low-cost carbon substrate. The structural properties of the biosurfactant and applicability of the biosurfactant in heavy metal removal were evaluated. Response surface methodology (RSM) involving central composite design (CCD) was used to optimize process parameters to maximize biosurfactant production. The study identified sugar cane molasses and sodium nitrate as carbon and nitrogen sources of choice for bacterial growth and biosurfactant production, with a relatively 2.64-fold increase in biosurfactant yield under optimized conditions. Analysis of the biosurfactant measured a surface tension reduction of water from 72.2 ± 0.26 to 30.5 ± 0.2 mN/m at 40 mg/L critical micelle concentration. GC–MS and FTIR analysis revealed structural properties of the lipopeptide biosurfactant, with fatty acid components predominantly 9-octadecenoic acid (oleic acid), n-hexadecanoic acid, cyclotetrasiloxane and trimyristin, and infrared peaks belonging to amine, carboxyl, nitrile, alkanol, ether and carbonyl groups. Capture of heavy metals using the biosurfactant was evaluated in soil microcosms. Removal rates of 80.47, 100, 77.57, 100, and 97.57% were recorded for As, Pb, Hg, Cd and Cr respectively after 12 weeks of incubation. There was no significant difference (p < 0.05) in the removal efficiency of the biosurfactant and an analogous chemical surfactant, sodium dodecyl sulphate. First and second-order kinetic models described heavy metal removal rates by the biosurfactant. We demonstrate the production of a useful biosurfactant using low-cost waste carbon.

Challenges of Green Transition in Polymer Production: Applications in Zero Energy Innovations and Hydrogen Storage

The green transition in the sustainable production and processing of polymers poses multifaceted challenges that demand integral comprehensive solutions. Specific problems of presences of toxic trace elements are often missed and this prevents shifting towards eco-friendly alternatives. Therefore, substantial research and the development of novel approaches is needed to discover and implement innovative, sustainable production materials and methods. This paper is focused on the most vital problems of the green transition from the aspect of establishing universally accepted criteria for the characterization and classification of eco-friendly polymers, which is essential to ensuring transparency and trust among consumers. Additionally, the recycling infrastructure needs substantial improvement to manage the end-of-life stage of polymer products effectively. Moreover, the lack of standardized regulations and certifications for sustainable polymers adds to the complexity of this problem. In this paper we propose solutions from the aspect of standardization protocols for the characterization of polymers foreseen as materials that should be used in Zero Energy Innovations in Hydrogen Storage. The role model standards originate from eco-labeling procedures for materials that come into direct or prolonged contact with human skin, and that are monitored by different methods and testing procedures. In conclusion, the challenges of transitioning to green practices in polymer production and processing demands a concerted effort from experts in the field which need to emphasize the problems of the analysis of toxic ultra trace and trace impurities in samples that will be used in hydrogen storage, as trace impurities may cause terrific obstacles due to their decreasing the safety of materials. Overcoming these obstacles requires the development and application of current state-of-the-art methodologies for monitoring the quality of polymers during their recycling, processing, and using, as well as the development of other technological innovations, financial initiatives, and a collective commitment to fostering a sustainable and environmentally responsible future for the polymer industry and innovations in the field of zero energy applications.

Sustainable production of multimeric and functional recombinant human adiponectin using genome-edited chickens

BackgroundAdiponectin (ADPN) plays a critical role in endocrine and cardiovascular functions, but traditional production methods, such as Escherichia coli and mammalian systems, have faced challenges in generating sufficiently active middle molecular weight (MMW) and high molecular weight (HMW) forms of recombinant human ADPN (hADPN). In our previous study, we proposed genome-edited chickens as an efficient platform for producing multimeric hADPN. However, the consistency of multimeric hADPN expression in this system across generations had not been further investigated.ResultsIn this study, subsequent generations of ovalbumin (OVA) ADPN knock-in chickens showed stable multimeric hADPN production, yielding ~ 26% HMW ADPN (0.59 mg/mL) per hen. Comparative analysis revealed that egg white (EW)-derived hADPN predominantly consisted of hexameric and HMW forms, similar to serum-derived hADPN. In contrast, hADPN obtained from human embryonic kidney (HEK) 293 and High-Five (Hi-5) cells also exhibited the presence of trimers, indicating variability across different production systems. Furthermore, transcriptional expression analysis of ADPN multimerization-associated endoplasmic reticulum chaperone genes (Ero1-Lα, DsbA-L, ERP44, and PDI) indicated upregulation in the oviduct magnum of ADPN KI hens, suggesting the chicken oviduct magnum as the optimal site for HMW ADPN production. Lastly, the functional analysis demonstrated that EW-derived hADPN significantly reduced lipid droplets and downregulated lipid accumulation-related genes (LOX-1, AT1R, FAS, and FABP4) in human umbilical vein endothelial cells (HUVECs).ConclusionIn summary, stable and functional multimeric hADPN can be produced in genome-edited chickens even after generations. This highlights the potential of using chicken bioreactor for producing various high-value proteins.